Insertion junction: LMJ.RY0402.177908_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.177908
Chromosome:chromosome 17
Location:1443497
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g706700 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCACTGCTGCACAACGCATATGCCATGAC
Internal bar code:GTATTTGCCGAATGAGTCTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:701
LEAP-Seq percent confirming:99.6527
LEAP-Seq n confirming:8609
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGGCTGTAAGTATCGGA
Suggested primer 2:GCTGCATCTACAGCCACGTA