| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177911 |
| Chromosome: | chromosome 1 |
| Location: | 1710181 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009150 | (1 of 1) PF14329 - Domain of unknown function (DUF4386) (DUF4386) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAGGTGAGCCCCAGAAGGCACCCGCAG |
| Internal bar code: | CTGGGCTAAGGTGAAAAGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 58.874 |
| LEAP-Seq n confirming: | 6034 |
| LEAP-Seq n nonconfirming: | 4215 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCAACTCTGTGATGGAA |
| Suggested primer 2: | AGACATCTCTCCATCCACCG |