| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177944 |
| Chromosome: | chromosome 2 |
| Location: | 6151585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGAGTCGCGCGTGCCAACACCTAGCC |
| Internal bar code: | TCTCGGCACTCCACACCTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1057 |
| LEAP-Seq percent confirming: | 99.7967 |
| LEAP-Seq n confirming: | 2945 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACACGGGTAGGTGTTTGA |
| Suggested primer 2: | GTAGTTGAGCGAGGTCAGGC |