| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177948 |
| Chromosome: | chromosome 14 |
| Location: | 1720171 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619500 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGTGCTGGACGTTGATGCTTCTTTCGA |
| Internal bar code: | CAGTCAGCACGCTACAAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 94.1801 |
| LEAP-Seq n confirming: | 2929 |
| LEAP-Seq n nonconfirming: | 181 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACACCTCCAACAATCAA |
| Suggested primer 2: | ACAGGCCCCAGTCAAACTC |