| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177982 |
| Chromosome: | chromosome 3 |
| Location: | 6694890 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197450 | (1 of 1) PTHR10799:SF676 - WINGED HELIX DNA-BINDING DOMAIN-CONTAINING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCCAGTTGCATGCAAATGGGGACCTTC |
| Internal bar code: | TACTGTCAGTTAGACGTTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTACCCTGAAGGAGTCAG |
| Suggested primer 2: | AGTATATGGTAGGCCGCACG |