| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.178010 |
| Chromosome: | chromosome 6 |
| Location: | 1348610 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258650 | PGM6 | Phosphoglycerate mutase-like protein; (1 of 4) PTHR23029:SF1 - FRUCTOSE-2,6-BISPHOSPHATASE TIGAR | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGCCGTACGGGCTATCGCAGAACCCAC |
| Internal bar code: | TAATCACCCGACAGCAGGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 191 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTCAATCACAGGTTTGC |
| Suggested primer 2: | GCAGTTTGAGCTTGGAAAGG |