| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178018 |
| Chromosome: | chromosome 10 |
| Location: | 1298297 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427453 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAATCGGCAGTGAAATCGGCGCATCGCT |
| Internal bar code: | TAAAGCCGTTCCTCAGGGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 94.9178 |
| LEAP-Seq n confirming: | 635 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGCAAAAGGCTTGGCTA |
| Suggested primer 2: | TGTATTAACTGCTGGCGCTG |