| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178035 |
| Chromosome: | chromosome 7 |
| Location: | 5597889 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351850 | NAT21 | (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTCCTGGCACCTCCCCGCCCACCCACTG |
| Internal bar code: | TGGTTGACGATGATTTGTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 99.0909 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAAGCACAGCCTGTATCG |
| Suggested primer 2: | CTACCCCGAGCAGAAGAGTG |