| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178049 |
| Chromosome: | chromosome 16 |
| Location: | 1414144 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652250 | CMA3 | Chlamydomonas-specific membrane protein family A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCAATCCAGTTTGGGGCAATTATCGACA |
| Internal bar code: | TTCCCACGGACCCAGCCGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 97.4252 |
| LEAP-Seq n confirming: | 3746 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGATCAGCCAGACGCAAG |
| Suggested primer 2: | GCTAGTGTGCCGACTGTCAA |