| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178051 |
| Chromosome: | chromosome_9 |
| Location: | 4738863 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g397142 | TRP15 | Transient receptor potential ion channel protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGAGGCGATGAGAGAGGCGGGGGACGGAGG |
| Internal bar code: | AAAGACGATTGTAGAGATGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 65.2715 |
| LEAP-Seq n confirming: | 577 |
| LEAP-Seq n nonconfirming: | 307 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTGAGCAGAAGTTAAGG |
| Suggested primer 2: | CTTTGGCTGGGTTTGGTTTA |