| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178073 |
| Chromosome: | chromosome 2 |
| Location: | 866225 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079150 | (1 of 1) PF03031//PF13671 - NLI interacting factor-like phosphatase (NIF) // AAA domain (AAA_33) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGCCATGCTAGAGGGCAGGCTGGGGA |
| Internal bar code: | CGAGTGGTGAATCGCTTCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 571 |
| LEAP-Seq percent confirming: | 99.4994 |
| LEAP-Seq n confirming: | 1590 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGCTTCTGAGGTACAGC |
| Suggested primer 2: | GGATCATTGTACTCCACCCG |