| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178080 |
| Chromosome: | chromosome 13 |
| Location: | 1882626 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575750 | (1 of 1) 2.5.1.105 - 7,8-dihydropterin-6-yl-methyl-4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase / Dihydropteroate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCTTGGTCGGGTAGCGTGCCGTTGCAC |
| Internal bar code: | GCCCGACCAATTAATAGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 99.7114 |
| LEAP-Seq n confirming: | 691 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACCCGCAGAGTCTGTTC |
| Suggested primer 2: | TGTGGAGCATTTCTGCAGTC |