Insertion junction: LMJ.RY0402.178081_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.178081
Chromosome:chromosome 9
Location:3246786
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g387763 (1 of 1) 1.1.1.94 - Glycerol-3-phosphate dehydrogenase (NAD(P)(+)) / L-glycerol-3-phosphate:NAD(P) oxidoreductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTTGCAAGCCAACGACGCATCTGCACAC
Internal bar code:ACGGGGAAGTCAAGTATCGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:356
LEAP-Seq percent confirming:54.6392
LEAP-Seq n confirming:424
LEAP-Seq n nonconfirming:352
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGGATGAGTTCGGATTGACA
Suggested primer 2:ATCGACCTTGGTCCACTCAC