Insertion junction: LMJ.RY0402.178083_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.178083
Chromosome:chromosome_14
Location:2463001
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g624950 DHC10,IDA2,DYH1B Flagellar inner arm dynein 1 heavy chain beta antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CCTCAACCTGGCATCCACCCTCGCATAATA
Internal bar code:CGGAAGCTGAGGGGGCTCACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:713
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1072
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGTCGTGTGAGAAATGGG
Suggested primer 2:CGCAAAAGAAGGAGAAGTGG