Insertion junction: LMJ.RY0402.178095_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.178095
Chromosome:chromosome 10
Location:3660439
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g445800 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACGGGTAGCGAATGTCGTGATTCACAT
Internal bar code:CTATTCCGTGGCGTGCACTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:302
LEAP-Seq percent confirming:99.5349
LEAP-Seq n confirming:2782
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGAAGCTCTCCACCATGC
Suggested primer 2:CACTACGCCAGCCTACACAA