| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178102 |
| Chromosome: | chromosome 6 |
| Location: | 2924535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272600 | (1 of 1) PF01429 - Methyl-CpG binding domain (MBD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCAGTTTTAACACTTGGGCAAGCTTTC |
| Internal bar code: | ATGGAACTCTGAGGAAAACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 99.5963 |
| LEAP-Seq n confirming: | 7402 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCACCGTACTGGGCACT |
| Suggested primer 2: | AACGGTACGATAAGGATGCG |