| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178105 |
| Chromosome: | chromosome 2 |
| Location: | 5708626 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110050 | (1 of 1) PTHR22844:SF160 - WD40 REPEAT-CONTAINING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCAGCGGGTCAGGGTTTCGAGCTGTGC |
| Internal bar code: | CCTTGAGATCGGTTCGTCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 275 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATACAGCACACGCTGTCG |
| Suggested primer 2: | ACCAGATGCTGCTCAAAGGT |