Insertion junction: LMJ.RY0402.178157_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.178157
Chromosome:chromosome 6
Location:7498333
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g300450 (1 of 14) IPR003593//IPR003959//IPR027417 - AAA+ ATPase domain // ATPase, AAA-type, core // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTGACACCTGCGTGCATGTGTGTGTGTG
Internal bar code:ATGCGGTTATTCTTTCTCATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:6
LEAP-Seq percent confirming:98.1665
LEAP-Seq n confirming:2570
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCCAATGTTGTCACGCTG
Suggested primer 2:CTTCCTCATTCAGCTCCTCG