| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178199 |
| Chromosome: | chromosome 2 |
| Location: | 250604 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074850 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCGGCCCCCCCTCTGAATCGCTGCGCT |
| Internal bar code: | CTCGTCTGAGCGACGAACGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 97.1368 |
| LEAP-Seq n confirming: | 916 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACCGCTGTGTTACGTGC |
| Suggested primer 2: | TGCATAGCTTGGATGCGTAG |