| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178247 |
| Chromosome: | chromosome 17 |
| Location: | 4958905 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734628 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGTTCCCACGAGGTTCGACTCGCCAC |
| Internal bar code: | TCTTCCTGGGCGGTCCCCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 99.0997 |
| LEAP-Seq n confirming: | 2752 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACATCAACCCACAGCAC |
| Suggested primer 2: | GCAGTATCACGCTGTCCTCA |