Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.178254 |
Chromosome: | chromosome 9 |
Location: | 4964196 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g397882 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGGCCAGTAGGGTGCTGATGCCCACCA |
Internal bar code: | TGGGCCTTGAATTGGGTTGATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 172 |
LEAP-Seq percent confirming: | 97.9949 |
LEAP-Seq n confirming: | 11192 |
LEAP-Seq n nonconfirming: | 229 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTCCGCTAGTTTGTCTTGG |
Suggested primer 2: | AACTGCAAACGCTCACACAC |