Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.178258 |
Chromosome: | chromosome_16 |
Location: | 6898001 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g676533 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGGGTCGGGGCCGCCATCGGTTGCGCTCA |
Internal bar code: | AATGCAATGATCTCCCAACGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 279 |
LEAP-Seq percent confirming: | 99.7453 |
LEAP-Seq n confirming: | 4699 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCTTGGAGGTCACACCGTT |
Suggested primer 2: | GAGGTGGTGATGCTGGTTCT |