| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178275 |
| Chromosome: | chromosome 17 |
| Location: | 694623 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700750 | GFY3 | (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCTCCGATGGCGCCAAGACCAAGGCCC |
| Internal bar code: | GTTGAGGGGGGGAGGGTAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 923 |
| LEAP-Seq percent confirming: | 96.7535 |
| LEAP-Seq n confirming: | 2712 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATACAGTGCAGCGGACAG |
| Suggested primer 2: | CTTGTTGGTCCAGGTCAGGT |