Insertion junction: LMJ.RY0402.178289_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.178289
Chromosome:chromosome 6
Location:5360835
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g283550 CRC2,POC6 Proteome of centriole protein 6; (1 of 1) K16466 - centrin-3 (CETN3, CDC31) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAAGGGCAAGCGCACTGTTCTTGTCCTTG
Internal bar code:TGCGTGTGGTCGTCAAAATGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1046
LEAP-Seq percent confirming:99.6554
LEAP-Seq n confirming:2892
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCCTTCATCCATTCGTC
Suggested primer 2:ACAGATGTCAGAGCACACGC