| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178299 |
| Chromosome: | chromosome 12 |
| Location: | 8609802 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547150 | (1 of 1) 3.5.3.18 - Dimethylargininase / N(G),N(G)-dimethylarginine dimethylaminohydrolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGTGCACTCGCGCTCGAGCCTCCACCA |
| Internal bar code: | CTAGAGGCATTCGGGGCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1027 |
| LEAP-Seq percent confirming: | 99.4522 |
| LEAP-Seq n confirming: | 2360 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCACCAACCCAGAACCT |
| Suggested primer 2: | CATGACCAGGAGATGTGTGG |