| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178323 |
| Chromosome: | chromosome 4 |
| Location: | 2714146 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223400 | (1 of 11) PF13229 - Right handed beta helix region (Beta_helix) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAACGTTAACCCCCCCCCCGCACCTGC |
| Internal bar code: | CCCATTTCGAAGTAGCACGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 99.7581 |
| LEAP-Seq n confirming: | 1237 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGGTGTAGAATGAGGTG |
| Suggested primer 2: | AACAGCAACAGCAACAGCAG |