| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178333 |
| Chromosome: | chromosome 12 |
| Location: | 6341931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537371 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACTGCATTGGTCGGTTGGGTTCCGTAT |
| Internal bar code: | AACTACAATATTTGTCGAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1463 |
| LEAP-Seq percent confirming: | 98.7928 |
| LEAP-Seq n confirming: | 982 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACTTGTCGGCACAGTAA |
| Suggested primer 2: | TGGATTGAGACTCTGTTGCG |