| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.178381 |
| Chromosome: | chromosome 12 |
| Location: | 3189619 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCACGGTCACACCCCACCTTCATGCCT |
| Internal bar code: | GGTATAAGAACTTGCAAGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 98.7179 |
| LEAP-Seq n confirming: | 1771 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGGCATAGAGACTACGA |
| Suggested primer 2: | GTCCGCGAAGTAGCCTACAG |