| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178384 |
| Chromosome: | chromosome 11 |
| Location: | 2024956 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468383 | (1 of 1) PTHR22844//PTHR22844:SF176 - F-BOX AND WD40 DOMAIN PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTAGTTGCTCCCAGGAGTGGGGCCGCC |
| Internal bar code: | TCCCCTTTAGCAAGTACACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATCGCCACAAGGATACG |
| Suggested primer 2: | TCGGATTCAGAGGAAGAGGA |