| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178384 |
| Chromosome: | chromosome 14 |
| Location: | 3173066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629000 | CGLD19 | Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTCGTTGCCTGCACGCCTTTGAAAGCAA |
| Internal bar code: | GAAGGGAAGGAGAAGCGCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 244 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTATCGTTTTCATGCTCG |
| Suggested primer 2: | GGTGTATGGAAGAGGAGGCA |