| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178397 |
| Chromosome: | chromosome 12 |
| Location: | 2977038 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCACGTGGTGGCATGCCCTTTGTCAGGT |
| Internal bar code: | GGGCACAGAACCCGGGCCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 99.8208 |
| LEAP-Seq n confirming: | 557 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGCCGAAAGTCAGTTCTT |
| Suggested primer 2: | AGGGGGCAACGTACATACAC |