| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178428 |
| Chromosome: | chromosome_1 |
| Location: | 2649348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g015500 | CGL91 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCTTGCCGAACCAGTCCTGGTCCTTGAGG |
| Internal bar code: | GCGCACATATACACCTGACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 995 |
| LEAP-Seq percent confirming: | 99.4479 |
| LEAP-Seq n confirming: | 2882 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACAGGGTGGAAGGTGTG |
| Suggested primer 2: | CTTTGCAACGCTGTAAGCAG |