| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178432 |
| Chromosome: | chromosome 16 |
| Location: | 4010727 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGCCGTCAGGTTATGCGGCTCCATGC |
| Internal bar code: | GAGGGGGTTGGAAACGTACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 99.7106 |
| LEAP-Seq n confirming: | 2067 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGGAACGCATGTGATGT |
| Suggested primer 2: | GCGTCCATACACGACTTCCT |