| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178448 |
| Chromosome: | chromosome 10 |
| Location: | 1622940 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429702 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGAGTTATATGGCGTTATGACTTCACAT |
| Internal bar code: | CATTGCCGAATGGATCGTGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 98.4192 |
| LEAP-Seq n confirming: | 2179 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTGATGCAGCTCCTGTA |
| Suggested primer 2: | GCATGGTGCAATAATGTTCG |