Insertion junction: LMJ.RY0402.178448_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.178448
Chromosome:chromosome 12
Location:8420308
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g548550 (1 of 1) PF05742 - Transport and Golgi organisation 2 (TANGO2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTCTGGCCATAGCAAGCACACGGACAGC
Internal bar code:TGGCAGAACTCGGGATGGTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:844
LEAP-Seq percent confirming:99.6693
LEAP-Seq n confirming:9646
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CATCTGCTACAGCAATCCGA
Suggested primer 2:GGCACCTCTACACCCCTACA