| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178450 |
| Chromosome: | chromosome 9 |
| Location: | 4839436 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397549 | (1 of 1) PF06972 - Protein of unknown function (DUF1296) (DUF1296) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGTGCGCAGAGGTACACACAGATTAGT |
| Internal bar code: | GTAGTCTCGGCCCCAACCGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 99.5964 |
| LEAP-Seq n confirming: | 4689 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATAACAACCCGACAACGC |
| Suggested primer 2: | GGGGAAAGAGACAGGAAAGG |