Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.178482 |
Chromosome: | chromosome_12 |
Location: | 4412800 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g520950 | BOP5,DIC4,IC138 | Flagellar inner dynein arm I1 intermediate chain IC138 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGCCACATGGGACCCGTGTACCAGGTGC |
Internal bar code: | GTATACTACGCAGCTGGGGTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 831 |
LEAP-Seq percent confirming: | 98.896 |
LEAP-Seq n confirming: | 3852 |
LEAP-Seq n nonconfirming: | 43 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGACTTCTCCACCTACTCGC |
Suggested primer 2: | CCACCTCACACTCAGCTCAA |