| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178486 |
| Chromosome: | chromosome 17 |
| Location: | 1843023 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709850 | ACK2 | (1 of 2) 2.7.2.1 - Acetate kinase / AK; Acetate kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGCATTGCCCGCCGCGCACCGCCGCCA |
| Internal bar code: | GGTCATCTTCGGTTCGGGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 99.6521 |
| LEAP-Seq n confirming: | 4583 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTACGGACTGCCATACG |
| Suggested primer 2: | GCCGTACAAGCTTATTCCCA |