| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.178496 |
| Chromosome: | chromosome 7 |
| Location: | 3314515 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334650 | (1 of 1) K10695 - E3 ubiquitin-protein ligase RNF1/2 [EC:6.3.2.19] (RNF1_2) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCAGGTAGACACGCGTGTTGTGCAGTA |
| Internal bar code: | CTGAACTCCCTCCAGGGTTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 18.2203 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 193 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTGCTGGGTGTTTTCTA |
| Suggested primer 2: | GCGAGGTAGGCTGTCGTAAG |