Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.178497 |
Chromosome: | chromosome_14 |
Location: | 780620 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g612950 | CYG17 | Adenylate/guanylate cyclase | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCGCGCCATGGAACTGCGCGGGCGCGGCAT |
Internal bar code: | ACGCGATTCCCAGCGGATGAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 985 |
LEAP-Seq percent confirming: | 99.8938 |
LEAP-Seq n confirming: | 3762 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGAGGGTACCAGCAAGGAG |
Suggested primer 2: | TCCTTTTAGGTTGGTGGTGC |