| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178508 |
| Chromosome: | chromosome 9 |
| Location: | 3432085 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389208 | (1 of 2) PTHR11266//PTHR11266:SF17 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // PROTEIN MPV17 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACACTGTGGGCGGTGAGGTCTGGCGTG |
| Internal bar code: | CGAGAGTTCCTAGGTTGTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 99.6898 |
| LEAP-Seq n confirming: | 18318 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAACGGTTTGGTCAAGTG |
| Suggested primer 2: | GACGATGGACGTGTTTGTTG |