| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178517 |
| Chromosome: | chromosome 16 |
| Location: | 2036792 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656950 | (1 of 1) PTHR13140:SF300 - PROTEIN TAG-278-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATATGCTTGCCCAGCATGCGCACGGGTG |
| Internal bar code: | TCAGCGCTCCGACCGTGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.6 |
| LEAP-Seq n confirming: | 498 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATCACCCTGCACATGAC |
| Suggested primer 2: | ATGCAAGGAGACGAGCAAGT |