| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178531 |
| Chromosome: | chromosome 3 |
| Location: | 3039605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164200 | (1 of 1) IPR023381 - YP001051499.1-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCGAAGGCGGCGGAGCGCTTCATGGGC |
| Internal bar code: | TCCCCGAGTTGATAAAGGGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 99.2462 |
| LEAP-Seq n confirming: | 790 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCTAGCGTGGTGACTGC |
| Suggested primer 2: | CCTATCTACCGCTCTGACGC |