Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.178539 |
Chromosome: | chromosome 2 |
Location: | 1515660 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g084300 | COQ5,UMM2,COQ5C,MENG | (1 of 1) K03183 - demethylmenaquinone methyltransferase / 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase (ubiE); Phylloquinone biosynthesis methyltransferase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCAACGTGTTGCCGTGGCTCACTGACG |
Internal bar code: | ACAACGGCCCTCTACGGAGGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 988 |
LEAP-Seq percent confirming: | 99.7633 |
LEAP-Seq n confirming: | 843 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGCACGTAATGCGTGTGTT |
Suggested primer 2: | CTGTTCCGGAGCTAGGTGTC |