Insertion junction: LMJ.RY0402.178547_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.178547
Chromosome:chromosome 17
Location:418551
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g698903 (1 of 1) IPR000014//IPR002395 - PAS domain // HMW kininogen CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCTGGTGATGTCGCTCATGCTGTCAGCG
Internal bar code:GCTTGTGCCGGCCAGGTCGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:307
LEAP-Seq percent confirming:23.0769
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGTAAGTAACTCCGTCGC
Suggested primer 2:ACACACACACACACACACGC