Insertion junction: LMJ.RY0402.178551_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.178551
Chromosome:chromosome 17
Location:5310053
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g736300 (1 of 1) IPR000104//IPR011047//IPR018391 - Antifreeze protein, type I // Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGATTGAAGCACCTCAATTGAATCATAA
Internal bar code:TGGCACTGGGTGTATTTCGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:300
LEAP-Seq percent confirming:99.2126
LEAP-Seq n confirming:126
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTTTGGTTTAGTCTGGGC
Suggested primer 2:TACGGCTACTGCTGCTGCTA