| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178562 |
| Chromosome: | chromosome 3 |
| Location: | 1542164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152300 | (1 of 2) IPR027414 - Glycosyl hydrolase family, N-terminal domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCCATGGAATCGTGCGCGGCCGAAAGC |
| Internal bar code: | GGGAGTAATGTTTCGCAGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 99.7773 |
| LEAP-Seq n confirming: | 448 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGATGGCTGGTACAGTAT |
| Suggested primer 2: | ATAAGCCGTGCAAGATACGG |