Insertion junction: LMJ.RY0402.178568_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.178568
Chromosome:chromosome_14
Location:2281368
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g623300 NCP1,NCPR1,NCR1 NADPH--cytochrome P450 reductase-related protein antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCTACGTCCACTCAGTTGTGTGCATCTGC
Internal bar code:CTTGTGTCTAATCTTGGATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:47
LEAP-Seq percent confirming:99.4413
LEAP-Seq n confirming:178
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTGGACTGCTCCTTCCAC
Suggested primer 2:GAGATAGGGGGAAGCCACTC