| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178573 |
| Chromosome: | chromosome 2 |
| Location: | 1516693 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084300 | COQ5,UMM2,COQ5C,MENG | (1 of 1) K03183 - demethylmenaquinone methyltransferase / 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase (ubiE); Phylloquinone biosynthesis methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAACAACTGCACGGACCCCATCCCGGAC |
| Internal bar code: | ATTGGTGGTCATTTCCCCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1294 |
| LEAP-Seq percent confirming: | 99.5539 |
| LEAP-Seq n confirming: | 5356 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTATCCACAAACCCACTT |
| Suggested primer 2: | GGCTTTGGTCTTTTAAGGGC |