| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178574 |
| Chromosome: | chromosome 7 |
| Location: | 2320304 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327950 | MAW9 | Membrane-associated hydroxyproline-rich glycoprotein 9 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTACGGCAACAGCGACACGGGCACAGC |
| Internal bar code: | GATGTTAGCCATTCTTCCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 99.8943 |
| LEAP-Seq n confirming: | 945 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCACGTAGGAGCAGACG |
| Suggested primer 2: | GTAACGCCTACGACGGCTAC |